Abstract—This study aimed to assess the effects of different concentrations (0, 1, 5, 10, 50 and 100 μg/mL) of the peppermint (Mentha piperita) extract on the motility and oxidative profile of rabbit spermatozoa following 0, 2, 4 and 8h of in vitro culture. Sperm motion was assessed using Computer aided sperm analysis, while Reactive Oxygen Species (ROS) production and the total antioxidant capacity were assessed by chemiluminescence. Protein and lipid oxidation were evaluated using spectrophotometric assays. The experiments revealed that low peppermint concentrations (1-10 μg/mL) exhibited motility-promoting effects (P<0.05 and P<0.01; 8h). Peppermint concentrations ranging between 1 and 50 μg/mL led to a significant preservation of the intracellular oxidative balance of rabbit spermatozoa (P<0.01; P<0.001; 8h). All selected peppermint concentrations prevented oxidative degeneration of proteins and lipids (P<0.05; P<0.01; P<0.001; 8h). Our results indicate that the peppermint extract could delay oxidative damage inflicted to spermatozoa by the in vitro environment.