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<i>In-Vitro</i> Propagation of Cinnamon (<i>Cinnamomum verum</i> Presl.) Using Embryos and <i>in Vitro</i> Axillary Buds

S. Subasinghe, C. S. Hettiarachchi, and N. Iddagoda
Department of Crop Science, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya, Sri Lanka
Abstract—Embryo culture was developed in half strength MS medium in order to optimize the culture condition for axillary buds. 15% Clorox® for 20 min was very effective in minimizing pathogenic contaminants (100% non-contaminants) embryos as well as minimum browning. Embryonic axis with ½ of cotyledon portion was used as explants for in-vitro establishment and inoculated into the half strength MS basal medium supplemented with 1.5mg L-1 BAP + 0.2mg L-1 IAA to initiate in-vitro seedlings, giving maximum culture initiation (90%). 1g L-1 activated charcoal was effective for establishment of in-vitro culture, recording minimum browning effect (34.9 mean rank value on non-browning appearance), enhancing stem elongation (19.5mm height) and leaf initiation (2.06 leaves/plantlet) after 14 days of culturing. Treatment combination of 0.1mg L-1 NAA + 4.0m L-1 BAP + 1.0g L-1 activated charcoal in full strength MS medium was effective for adventitious root elongation on in-vitro micro-stem cuttings and given the highest root length (6.7cm) after 6 weeks of incubation period. Coir dust was the best potting medium for acclimatization giving maximum survival (90%). Therefore, the findings of the research could be used as a protocol for in-vitro propagation of Cinnamon (Cinnamomum verum Presl.) through in vitro axillary buds. 
Index Terms—axillary BUDS, Cinnamomum verum Presl., in-vitro propagation

Cite: S. Subasinghe, C. S. Hettiarachchi, and N. Iddagoda, "In-Vitro Propagation of Cinnamon (Cinnamomum verum Presl.) Using Embryos and in Vitro Axillary Buds," Journal of Advanced Agricultural Technologies, Vol. 3, No. 3, pp. 164-169, September 2016. Doi: 10.18178/joaat.3.3.164-169
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