Abstract—Embryo culture was developed in half strength MS medium in order to optimize the culture condition for axillary buds. 15% Clorox® for 20 min was very effective in minimizing pathogenic contaminants (100% non-contaminants) embryos as well as minimum browning. Embryonic axis with ½ of cotyledon portion was used as explants for in-vitro establishment and inoculated into the half strength MS basal medium supplemented with 1.5mg L^-1 BAP + 0.2mg L^-1 IAA to initiate in-vitro seedlings, giving maximum culture initiation (90%). 1g L^-1 activated charcoal was effective for establishment of in-vitro culture, recording minimum browning effect (34.9 mean rank value on non-browning appearance), enhancing stem elongation (19.5mm height) and leaf initiation (2.06 leaves/plantlet) after 14 days of culturing. Treatment combination of 0.1mg L^-1 NAA + 4.0m L^-1 BAP + 1.0g L^-1 activated charcoal in full strength MS medium was effective for adventitious root elongation on in-vitro micro-stem cuttings and given the highest root length (6.7cm) after 6 weeks of incubation period. Coir dust was the best potting medium for acclimatization giving maximum survival (90%). Therefore, the findings of the research could be used as a protocol for in-vitro propagation of Cinnamon (Cinnamomum verum Presl.) through in vitro axillary buds. 
 

Index Terms—axillary BUDS, Cinnamomum verum Presl., in-vitro propagation

Cite: S. Subasinghe, C. S. Hettiarachchi, and N. Iddagoda, "In-Vitro Propagation of Cinnamon (Cinnamomum verum Presl.) Using Embryos and in Vitro Axillary Buds," Journal of Advanced Agricultural Technologies, Vol. 3, No. 3, pp. 164-169, September 2016. Doi: 10.18178/joaat.3.3.164-169