Abstract—The effects of mine waste toxicity are very low acidity, high sulfuric ion and heavy metal contents. The aimed at studying and knowing the ability of SRB isolates in reducing sulfate ions, increasing pH and deposition of heavy metals in overburden and the ability of SRB in reducing the effects of mining waste toxicity on bioindicators. Compiled based on a complete randomized design of two factors. The first factor of SRB inoculum type, isolate K 06, K18, and K 28 and without SRB inoculum. The second factor: gamal, rice straw, blotong and manure. Parameters observed were pH, sulfate content, heavy metal content of Ni in overburden waste, water and bioindicators. The results reveal that the treatment of SRB K18 and gamal have the best effect on the growth of rice crops those are 63.25 cm high, 24.17 pieces of leaves, number of tiller 6.08 and dry weight of plant 21.48 g. While treatment without inoculum and blotong gave the lowest growth in plants. The decline of Ni content on overburden by SRB K18 and type of the lowest blotong is 1810.69 ppm. The lowest sulfate content of soil is 43.22 ppm treated with SRB K 28 with manure.