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Evaluation of 2.4-D and NAA Concentrations for Callus and Somatic Embryos Formation in Oil Palm

Reflini
Plant Production and Biotechnology Division, PT. SMART Tbk, Jakarta, Indonesia
Abstract—The evaluation of 2.4-D and NAA concentrations for oil palm micropropagation were conducted in this study. Callus was initiated from immature leaflet of ten oil palm trees. Callus formation began two months after culture and increased at the subsequent subcultures. On average, the best callus induction rate was obtained in a culture medium without 2.4-D in combination with 6 mg/L of NAA. After 11 months of culture, somatic embryos were found mostly from nodular aggregate and nodular friable callus. At the end of culture time, the results showed that 6 mg/L of NAA slightly increase somatic embryos formation by the addition of 0.5 mg/L 2.4-D in both explants and callus culture. When 2.4-D was only added during callus culture, the formation of somatic embryos was low. Different results were shown in treatment with 10 mg/L of NAA. The formation of somatic embryos were much better by the addition of 0.5 mg/L 2.4-D only during callus culture. When 0.5 mg/L 2.4-D was added during explants and callus culture, the formation of somatic embryos were very low. In general, treatment of 10 mg/L NAA added with 0.5 mg/L 2.4-D during callus culture was the best media for oil palm propagation.

Index Terms—auxin, plant growth regulators, somatic embryogenesis, micropropagation

Cite: Reflini, "Evaluation of 2.4-D and NAA Concentrations for Callus and Somatic Embryos Formation in Oil Palm," Journal of Advanced Agricultural Technologies, Vol. 4, No. 3, pp. 215-218, September 2017. Doi: 10.18178/joaat.4.3.215-218
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