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Filter Membrane Separation and Purification of Jatropha Protein Extraction Solution

Tsair-Wang Chung 1, Bo-Lin Chen 1, Shih-Hong Hsu 1, Yi-Feng Lin 1, and Yung-Sen Lin 2
1. Department of Chemical Engineering, Chung Yuan Christian University, Taoyuan 320, Taiwan
2. Department of Chemical Engineering, Feng Chia University, Taichung 407, Taiwan
Abstract—Jatropha curcas is a multiple purpose plant and its seeds contain about 30%-40% oil. The oil can be directly used as fuel or to be the raw material for biochemical industry. The protein is usually extracted from the pressed cake. As noted that oil content in the pressed cake will affect the protein extraction efficiency. After de-oil pretreatment of the pressed cake, the oil content can be reduced from 23% to 0.581%. The Response Surface Methodology (RSM) was used to get the optimal operating parameters for protein extraction from de-oil cake and further separation by the multistage membrane filtration process. The highest yield of protein was obtained at 30°C, with 0.1 M sodium hydroxide solution, and solid to liquid ratio of 1:12. The protein solution was further passed through a series of tubular membrane in different connections, which can get the purity of raw protein powders in 78.44% after drying.
Index Terms—membrane, filtration, Jatropha, protein

Cite: Tsair-Wang Chung, Bo-Lin Chen, Shih-Hong Hsu, Yi-Feng Lin, and Yung-Sen Lin, "Filter Membrane Separation and Purification of Jatropha Protein Extraction Solution," Journal of Advanced Agricultural Technologies, Vol. 4, No. 2, pp. 170-174, June 2017. Doi: 10.18178/joaat.4.2.170-174
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