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Mass Propagation of <i>Lagenaria siceraria</i> through <i>in Vitro</i> Culture

Nor A. Hasbullah 1, Mohammad M. Lassim 2, Muhammad A. Mazlan 1, Siti Z. Lood 1, and Muhamad A. Mohamed Amin 1
1. Department of Agriculture Science, Faculty of Technical and Vocational Eduation, Universiti Pendidikan Sultan Idris (UPSI), 35900 Tanjong Malim, Perak, Malaysia
2. Faculty of Sustainable Agriculture (Sandakan Campus), Universiti Malaysia Sabah (UMS), Locked Bag No. 3, 90509 Sandakan, Sabah, Malaysia
Abstract—This aim of this research is to study and investigate mass propagation of Lagenaria siceraria through tissue culture system. This plant is commonly known as ‘bottle guard’ or ‘calabash’ and belongs to the family Cucurbitaceae. It is commonly cultivated in the tropical and subtropical regions of the world. The plant is grown for its fruit, which usually harvested and consumed as vegetable. Complete plant regeneration was successfully achieved when aseptic stem explant was cultured on MS medium supplemented with various combinations of plant growth regulators. MS medium supplemented with 2.0 mg/L Benyzlaminopurine (BAP) and 0.5 mg/L Napthalene Acetic Acid (NAA) was found to be the optimum medium and stem explant was the most responsive explant producing 4.633 ± 0.084 shoots per explant. Basal MS medium (without hormone) was optimum for root induction. Established plantlets were later acclimatized to the green house for further growth and development process. This research was successfully achieved and could serve as a good alternative to crop production in order to maintain the food security chain all over the world.
 
Index TermsLagenaria siceraria, tissue culture, regeneration, MS medium, acclimatization

Cite: Nor A. Hasbullah, Mohammad M. Lassim, Muhammad A. Mazlan, Siti Z. Lood, and Muhamad A. Mohamed Amin, "Mass Propagation of Lagenaria siceraria through in Vitro Culture," Journal of Advanced Agricultural Technologies, Vol. 4, No. 1, pp. 92-95, March 2017. Doi: 10.18178/joaat.4.1.92-95
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