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Comparison of Vero and a New Suspension Cell Line in Propagation of Peste des Petits Ruminants Virus (PPRV)

Seyed Mohamad Javad Taher Mofrad 1, Mohsen Lotfi 2, and Masoud Parsania 3
1. Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
2. Quality Control Dep. Razi Vaccine and Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
3. Department of Microbiology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Abstract—Peste des Petits Ruminants (PPR) is an acute, highly contagious and world organization for animal health (OIE) notifiable economically important transboundary viral disease of sheep, goat and wild ruminant species with high morbidity and mortality and caused by PPR virus. Currently PPR control is done by vaccination with an attenuated PPR strain (Nigeria 75/1) produced in monolayers of Vero cells grown in roller bottles or static flasks. Vero cell culture is very time consuming and difficult. As an alternative, we introduce a new suspension cell line as cell substrate for virus propagation. After selection the most sensitive cell clones to the virus and adaptation on suspension cell line, growth characteristics of new cell substrate was evaluated. Also, the conditions of cell culture and virus culture were optimized, and finally titer of production was measured and compared to titer of PPR vaccine available. Sensitivity of cell to the virus was stable during many passages. The Average final titer of virus production was 6.7 log10 CCID50/ml and may be higher in bioreactor. 
 
Index Terms—PPR vaccine, Peste des petits ruminant’s disease, Suspension cell line, growth curve

Cite: Seyed Mohamad Javad Taher Mofrad, Mohsen Lotfi, and Masoud Parsania, "Comparison of Vero and a New Suspension Cell Line in Propagation of Peste des Petits Ruminants Virus (PPRV)," Journal of Advanced Agricultural Technologies, Vol. 3, No. 3, pp. 207-212, September 2016. Doi: 10.18178/joaat.3.3.207-212
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