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Effect of Feed Restriction and Dietary Fat Type on Liver Fatty Acid Binding Protein mRNA Expression in the Broiler Chickens

Bahman Navidshad 1 and Maryam Royan 2
1. Department of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran
2. Agricultural Biotechnology Research Institute of Iran- North Branch, Rasht, Iran
Abstract—Liver fatty acid binding protein (L-FABP) is the main cytosolic binding site for long chain fatty acids in hepatocytes. FABPs enhance uptake of fatty acids into the cell by increasing their concentration gradient, due to minimizing unbound fatty acid in the cell. A total of 720, 10-day old male Ross 308 broiler chicks were fed diets with unsaturated to saturated fatty acid ratio (U/S) of 2, 3.5, 5 or 6.5 as ad lib or skip a day feeding schedule (during 18-28 days of age). The results clearly showed that feed restriction induced L-FABP gene expression in the livers of broiler chickens. The L-FABP gene expression increased by dietary unsaturated to saturated fatty acid ratio of 6.5. No interaction of dietary U/S and feed restriction on the liver L-FABP gene expression was observed. This observation proposes that birds have a mechanism for regulation of fatty acids transfer under different nutritional condition. 
 
Index Terms—L-FABP, real time PCR, feed restriction, dietary U/S, broiler chickens

Cite: Bahman Navidshad and Maryam Royan, "Effect of Feed Restriction and Dietary Fat Type on Liver Fatty Acid Binding Protein mRNA Expression in the Broiler Chickens," Journal of Advanced Agricultural Technologies, Vol. 3, No. 1, pp. 15-19, March 2016. Doi: 10.18178/joaat.3.1.15-19
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