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Efficiency Evaluation of Vanda Tricolor Growth in Temporary Immerse System Bioreactor and Thin Layer Culture System

Rizkita R. Esyanti 1, Nikko Adhitama 2, and Robert Manurung 2
1. Department of Biology, School of Life Science and Technology, Institut Teknologi Bandung, Jln. Ganesha 10 Bandung 40132 Indonesia
2. Department of Bioengineering, School of Life Science and Technology, Institut Teknologi Bandung, Jln. Ganesha 10 Bandung 40132 Indonesia
Abstract—Vanda tricolor (aliases: Vanda suavis) is one of native orchid species found in Indonesia and surrounding countries in SE Asia. It is economically important due to its unique and fragrant smell of its flower. This unique properties increases the demand of this orchid. In order to match the consumer’s demand, one of the solutions to mass-produce V. tricolor is by using micropropagation method by thin layer liquid medium and temporary immerse system (TIS) bioreactor. In this study, shoot culture of V. tricolor is cultivated in full MS media with addition of coconut water using two different systems, thin layer and TIS bioreactor. Two different variations of immersion period of 5 and 10 minutes every 12 hours is given in culture in TIS bioreactor. After 21 days, both systems were analyzed to determine which system and parameter was efficient in accordance to visual and viability analysis, biomass production, and medium utilization (conductivity, and sugar consumption). The result showed that shoots culture in TIS bioreactors are more viable and visually better than in thin layer system. Thin layer system has the highest sugar utilization and conductivity decrease with the value of 4.854 g and 7.52 mS respectively. Largest biomass production occurs in thin layer system with specific growth rate of 0.056/day. Based on visual and viability analysis, biomass production, and medium utilization, this study conclude that TIS bioreactor is more efficient in producing V. tricolor shoot. 
 
Index Terms—immersion period, temporary immersion system, thin layer, vanda tricolor

Cite: Rizkita R. Esyanti, Nikko Adhitama, and Robert Manurung, "Efficiency Evaluation of Vanda Tricolor Growth in Temporary Immerse System Bioreactor and Thin Layer Culture System," Journal of Advanced Agricultural Technologies, Vol. 3, No. 1, pp. 63-66, March 2016. Doi: 10.18178/joaat.3.1.63-66
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