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Polymorphism of a Mutation of DGAT1 Gene in Lori Sheep Breed

Shahram Nanekarani, Mojtaba Kolivand, and Majid Goodarzi
Department of Animal Science, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran
Abstract—The objective of this study was to analyze exon 16 - 17 of DGAT1 gene polymorphism in Lori sheep breed. This Gene which is a key acyltransferase in triglyceride biosynthesis, has been located to chromosome 9 of sheep. A total of 118 blood samples have been collected from Lori sheep breed and genotypes were determined by PCR-RFLP assay. The amplified product was observed as 309 bp and the restriction digestion with AluI revealed three genotypes, TT (272 and 37 bp), TC (309, 272 and 37 bp) and CC (309 bp). The results showed that allele frequencies of the DGAT1 gene were 0.562 and 0.438 for C and T allele, respectively. Average heterozygosity for this locus was suitable (0.49). The genotype of SNP in population was not in consent with Hardy-Weinberg equilibrium (p<0.01). The results indicated that this indigenous sheep breed is proper polymorphism and could be used to guide association studies between this polymorphism and Carcass traits.
Index Terms—lori sheep, PCR-RFLP, DGAT1 gene, polymorphism

Cite: Shahram Nanekarani, Mojtaba Kolivand, and Majid Goodarzi, "Polymorphism of a Mutation of DGAT1 Gene in Lori Sheep Breed," Journal of Advanced Agricultural Technologies, Vol. 3, No. 1, pp. 38-41, March 2016. Doi: 10.18178/joaat.3.1.38-41
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