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Anticancer Activity of 3-Hydroxystigmastan- 5(6)-en (β-Sitosterol) Compound from Salacca Edulis Reinw Variety Bongkok in MCF-7 and T47D Cell Line

Leni Herliani Afrianti1, Willy Pranata Widjaja2, Neneng Suliasih2, Wahyu Widowati3, Nurul Fauziah4, Maesaroh Maesaroh4, and Pande Putu Erawijantari4
1. Pasundan University, Departement of Food Technology, Bandung, Indonesia
2. Departement of Food Technology, Pasundan University, Bandung, Indonesia
3. Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia
4. Bimolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia
Abstract—Discovery of two compounds in ethylacetate extract of snake fruits (Salacca edulis Reinw) variety Bongkok were pyrolle-2,4-dicarboxylic acid-methyl ester and 3-hydroxystigmastan-5(6)-en (β-sitosterol) compounds. In a previous study, two new compounds were observed an antioxidant activity by 2,2-diphenylpicrylhydrazyl (DPPH) free radical scavenging activity. Pyrolle-2,4-dicarboxylic acid-methyl ester posses cytotoxic activity against MCF7 and T47D cell line. 3-hydroxystigmastan-5(6)-en (β-sitosterol) from snake fruit ethyl acetate extract- potential as anticancer still remains unknown and will be observed in his study. The cytotoxic assay was performed using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2- (4-sul-fophenyl)-2H-tetrazolium) (MTS) method in MCF and T47D cell lines to determine the IC50 of the β-sitosterol from snake fruit extract. Cytotoxic assay revealed that the (β-sitosterol) compound of snake fruit (Salacca edulis Reinw) inhibits the proliferation and viability of MCF7-breast cancer line- (IC50= 45.414 μg/mL) and T47D -breast cancer stem cell line- (IC50 = 1.1942 μg/mL). The result obtained from the current study demonstrated that the β-sitosterol from snake fruit (Salacca edulis Renw.) extract exhibited in vitro cytotoxicity against MCF7 (breast cancer cell line) and T47D (breast cancer stem cell line).
 
Index Terms—anticancer, salacca edulis, β-sitosterol, breast cancer

Cite: Leni Herliani Afrianti, Willy Pranata Widjaja, Neneng Suliasih, Wahyu Widowati, Nurul Fauziah, Maesaroh Maesaroh, and Pande Putu Erawijantari, "Anticancer Activity of 3-Hydroxystigmastan- 5(6)-en (β-Sitosterol) Compound from Salacca Edulis Reinw Variety Bongkok in MCF-7 and T47D Cell Line," Journal of Advanced Agricultural Technologies, Vol. 2, No. 2, pp. 129-133, December 2015. Doi: 10.12720/joaat.2.2.129-133
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