Abstract—The microbial lipid was interested to be an alternative feedstock of biodiesel production. The lipid producing condition of oleaginous yeast Psuedozyma sp. was optimized. The response surface methodology (RSM) was applied with central composite design (CCD) in this study. The experiment results showed that a second-order polynomial regression equation was achieved with good coefficient of determination in analysis of variance. The validation tests were performed and the experimented results were not significant different to the predicted value, thus confirming the reliability of empirical model in describing lipid production. Moreover, the fatty acid compositions were investigated. The results revealed that the accumulated lipid mainly consisted of 85% in C16:C18 fatty acids. In addition, yeast biodiesel properties were predicted and also provided a satisfactory property. It was suggested that lipid of Psuedozyma sp. was suitable to be an alternative feedstock for biodiesel manufacturing.

Index Terms—oleaginous yeast, microbial biodiesel, response surface methodology, biodiesel property

Cite: Atsadawut Areesirisuk, Tsair-Bor Yen , Chiu-Hsia Chiu, Chun-Hung Liu, and Jia-Hsin Guo, "Optimization on Yeast Lipid Production of Psuedozyma sp. with Response Surface Methodology for Biodiesel Manufacturing," Journal of Advanced Agricultural Technologies, Vol. 2, No. 1, pp. 13-18, June 2015. Doi: 10.12720/joaat.2.1.13-18