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Potential of Pseudomonas Aeruginosa to Control Sclerotium Rolfsii Causing Stem Rot and Collar Rot Disease of Tomato

Natedara Chanutsa2, Nutchanat Phonkerd1, and Wandee Bunyatratchata2
1.Faculty of Applied Science and Engineering, Nong Khai Campus, Khon Kaen University, Nong Khai 43000, Thailand
2.Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
Abstract—The aim of this study was to isolate and select the highest potential activities of bacteria from 7 provinces in northeastern region of Thailand against Sclerotium rolfsii. Thirteen of 329 isolates were screened as antagonistic bacteria to inhibit S. rolfsii by dual culture assay. High percentages of inhibition were found in three isolates of UD1EBa-2, KK1EBa-3 and KK11EBa-3with 51.25%, 56.25% and 60.00%, respectively. The culture filtrate of each bacterium was prepared to test the mycelial growth inhibition of S.rolfsii. The results showed that the culture filtrate could inhibit mycelial growth with 100%. The most effective isolate KK11EBa-3 was characterized to be rod shape, gram negative and non spore forming. The colony on Nutrient agar was circular, smooth, translucent, flat elevation. Based on molecular sequencing data, it was identified as Pseudomonas aeruginosa.

Index Terms—Sclerotium rolfsii, Biocontrol, Antagonistic bacteria, culture filtrate

Cite: Natedara Chanutsa, Nutchanat Phonkerd, and Wandee Bunyatratchata, "Potential of Pseudomonas Aeruginosa to Control Sclerotium Rolfsii Causing Stem Rot and Collar Rot Disease of Tomato," Journal of Advanced Agricultural Technologies, Vol. 1, No. 2, pp. 132-135, December 2014. Doi: 10.12720/joaat.1.2.132-135
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